wave 3500 ht denaturing gradient hplc system Search Results


preparative plrp s polymeric c18 rp hplc column  (Agilent technologies)
98
Agilent technologies preparative plrp s polymeric c18 rp hplc column
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Preparative Plrp S Polymeric C18 Rp Hplc Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pmc07537637-180-35-47?v=Agilent+technologies
Average 98 stars, based on 1 article reviews
preparative plrp s polymeric c18 rp hplc column - by Bioz Stars, 2026-07
98/100 stars
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rp hplc  (ATCC)
92
ATCC rp hplc
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Rp Hplc, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/10__21608_slash_ejabf__2022__221762-84-45-96?v=ATCC
Average 92 stars, based on 1 article reviews
rp hplc - by Bioz Stars, 2026-07
92/100 stars
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vwr extra pure  (Chem Impex International)
95
Chem Impex International vwr extra pure
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Vwr Extra Pure, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pm20670021__ol101450u_si_001-34-28-32?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
vwr extra pure - by Bioz Stars, 2026-07
95/100 stars
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tandem mass spectrometry  (Danaher Inc)
96
Danaher Inc tandem mass spectrometry
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Tandem Mass Spectrometry, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pm38049233-62-9-15?v=Danaher+Inc
Average 96 stars, based on 1 article reviews
tandem mass spectrometry - by Bioz Stars, 2026-07
96/100 stars
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hplc-ed l-3500 detector  (Merck KGaA)
90
Merck KGaA hplc-ed l-3500 detector
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Hplc Ed L 3500 Detector, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pm38673988-393-36-41?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
hplc-ed l-3500 detector - by Bioz Stars, 2026-07
90/100 stars
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chip-based quadrupole coupled to hplc microsaic 3500 mid  (Microsaic Systems)
90
Microsaic Systems chip-based quadrupole coupled to hplc microsaic 3500 mid
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Chip Based Quadrupole Coupled To Hplc Microsaic 3500 Mid, supplied by Microsaic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pmc08932337-409-6-7?v=Microsaic+Systems
Average 90 stars, based on 1 article reviews
chip-based quadrupole coupled to hplc microsaic 3500 mid - by Bioz Stars, 2026-07
90/100 stars
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hplc pump 3500  (GE Healthcare)
92
GE Healthcare hplc pump 3500
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Hplc Pump 3500, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pmc00061157-107-1-4?v=GE+Healthcare
Average 92 stars, based on 1 article reviews
hplc pump 3500 - by Bioz Stars, 2026-07
92/100 stars
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model 7125 injector  (Rheodyne lp)
90
Rheodyne lp model 7125 injector
Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by <t>non-reductive</t> <t>RP-HPLC</t> and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner
Model 7125 Injector, supplied by Rheodyne lp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave+3500+ht+denaturing+gradient+hplc+system/pm12773037-105-25-49?v=Rheodyne+lp
Average 90 stars, based on 1 article reviews
model 7125 injector - by Bioz Stars, 2026-07
90/100 stars
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recombinant human cxcl9 mig protein  (Novus Biologicals)
N/A
The Recombinant Human CXCL9 MIG Protein from Novus Biologicals is derived from E coli The Recombinant Human CXCL9 MIG Protein has been validated for the following applications Functional SDS Page
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hypercarb high temperature hplc columns  (Thermo Fisher)
N/A
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recombinant human fabp6 protein  (Novus Biologicals)
N/A
The Recombinant Human FABP6 Protein from Novus Biologicals is derived from E coli The Recombinant Human FABP6 Protein has been validated for the following applications Functional SDS Page
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recombinant human ccl21 6ckine protein  (Novus Biologicals)
N/A
The Recombinant Human CCL21 6Ckine Protein from Novus Biologicals is derived from E coli The Recombinant Human CCL21 6Ckine Protein has been validated for the following applications Functional SDS Page
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Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by non-reductive RP-HPLC and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner

Journal: Glycoconjugate journal

Article Title: Recombinant human heterodimeric IL-15 complex displays extensive and reproducible N - and O -linked glycosylation

doi: 10.1007/s10719-015-9627-1

Figure Lengend Snippet: Overview of the expression, cellular presentation, function and analysis of the soluble human hetIL-15 complex. a. Engineered human IL-15 and IL-15Rα were co-expressed and secreted by HEK293 cells as a soluble heterodimeric complex (hetIL-15) after proteolytic cleavage from the cell surface. The complex binds to the IL-2Rβ/γ receptor complex located on target cells, where it initiates a cellular response. b. The hetIL-15 complex was isolated and its purity monitored using reducing (upper) and native (lower) SDS-PAGE with Coomassie blue staining (left gels) and Western blotting using anti-IL-15 and anti-IL-15Rα antibodies (right gels). The two clinically relevant preparations of hetIL-15 (i.e. the EN and cGMP lots) are shown. c. IL-15 and sIL-15Rα were separated from their heterodimeric complex by non-reductive RP-HPLC and individually subjected to LC-MS/MS-based glycan (top), glycopeptide (bottom) and glycoprotein (right) profiling in order to characterize their N- and O-glycosylation in a detailed and site-specific manner

Article Snippet: Slightly different conditions were used to separate the two examined hetIL-15 preparations ( i.e. , EN and cGMP lots): The EN lot of hetIL-15 was separated at a flow rate of 5 ml/min on a preparative PLRP-S polymeric C18 RP-HPLC column (i.d., 50 mm, length, 150 mm, Agilent).

Techniques: Expressing, Isolation, SDS Page, Staining, Western Blot, Liquid Chromatography with Mass Spectroscopy